Stable Transfection:Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXs-Puro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41-mCherry), or EBOV GP (pMXs-IN GP) as previously described [ ].
Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles.
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXsPuro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41mCherry), or EBOV GP (pMXs-IN GP) as previously described [73].
Expressing:Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXs-Puro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41-mCherry), or EBOV GP (pMXs-IN GP) as previously described [ ].
Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles.
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXsPuro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41mCherry), or EBOV GP (pMXs-IN GP) as previously described [73].
Cell Culture:Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5 ] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41-mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXs-Puro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41-mCherry), or EBOV GP (pMXs-IN GP) as previously described [ ].
Article Title: Platelet-like particles released from Ebola virus-infected megakaryocytic cells behave like virus-like particles.
Article Snippet: .. Wild-type MEG-01 cells (ATCC, CRL-2021), MEG-01 VP30 cells (MEG-01 cell line stably expressing EBOV VP30), MEG-01 CD41-mCherry cells (MEG-01 cell line stably expressing CD41 [GenBank accession no. NM_000419.5] fused with mCherry at the C-terminus [CD41-mCherry]), and MEG-01 CD41-mCherry/GP cells (MEG-01 cell line stably expressing CD41mCherry and EBOV GP) were cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and antibiotics. .. MEG-01 stable cell lines were generated by transducing cells with a pMXs-IRES-Neo (pMXs-IN; Cell Biolabs) or pMXsPuro (pMXs-IP; Cell Biolabs) retroviral vector encoding EBOV VP30 (pMXs-IN VP30), CD41-mCherry (pMXs-IP CD41mCherry), or EBOV GP (pMXs-IN GP) as previously described [73].
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